Environmental impacts and effects on greenhouse gas emissions in shrimp feed production system for aquaculture - A case study in India.

Attributional life cycle assessment study examines the environmental impact of raw materials, machinery, and unit operations. In the present work, an attributional life cycle assessment (LCA) was employed to assess the environmental and greenhouse gas impacts of a shrimp feed production system. A commercial shrimp feed mill in Tamil Nadu, India, provided inventory data for one-ton shrimp feed (functional unit) for a Cradle-to-Gate evaluation using environmental impact methodologies, specifically Impact 2002+ in SimaPro® (V9.3.0.3) software. The results showed that human health (0.003357 DALY), ecosystem quality (2720.518 PDF × m2 × yr), climate change (2031.696 kg CO2 eq), and resources (71019.42 MJ primary) were the most significantly impacted. The human health category was found to be the most prominent after normalization and weighting (0.47 pt), and strategies were suggested accordingly. The GWP20 and GWP100 measures for long-term climate change were calculated to be 8.7 and 7.33 kg CO2 eq, respectively. Cast iron used in machinery production (GWP 20-15.40%, GWP100-134.5%) and electricity use (GWP 20-6.13%, GWP 100-6.9%) accounted for sizable portions of the burden. Feed production is estimated to contribute 0.2% of global CO2 emissions within the proposed global context. These findings are significant regarding economically and environmentally sustainable shrimp feed production worldwide.

Neuropathy Dermatitis: An Underdocumented Complication Following Total Knee Arthroplasty.

Background: Midline surgical incision used in Total Knee Arthroplasty (TKA) is associated with iatrogenic injury to the infrapatellar branch of the saphenous nerve, which leads to neuropathic dermatitis around the healed surgical scar. There are very few studies with a limited number of cases that have reported this complication. We evaluated the incidence of neuropathic dermatitis and its implication for the functional outcome in TKA patients. Methodology: Patients who underwent primary TKA between 1 January 2010 and 31 August 2019 and presented in follow-up with sensory disturbances and skin lesions adjacent to the surgical incision were evaluated in this study. Results: A total of 3318 patients with 4282 TKAs were included, of which 188 patients presented with the clinical picture of neuropathic dermatitis. There were 136 females and 52 males with a mean age of 67.13 years (range 37-92 years). The mean duration from surgery to the appearance of skin lesions was 4.4 months (range 2-6 months), and they resolved at a mean duration of 7.67 (range 6-12) weeks. In our study, we found an incidence of 5.52%. All these patients had a stable and well-functioning knee at the time of presentation of the lesion with a mean Knee Society Score (KSS) of 92 (range 84-96). Conclusion: In our study, we found the incidence of neuropathic dermatitis to be 5.52%, without any long-term implication on the functional outcome of operated knees. For a self-limiting complication of midline knee incision of TKA, it either resolves on its own or requires a short duration of topical steroid application.

Ultraparamagnetic Cells Formed through Intracellular Oxidation and Chelation of Paramagnetic Iron.

Making cells magnetic is a long-standing goal of chemical biology, aiming to enable the separation of cells from complex biological samples and their visualization in vivo using magnetic resonance imaging (MRI). Previous efforts towards this goal, focused on engineering cells to biomineralize superparamagnetic or ferromagnetic iron oxides, have been largely unsuccessful due to the stringent required chemical conditions. Here, we introduce an alternative approach to making cells magnetic, focused on biochemically maximizing cellular paramagnetism. We show that a novel genetic construct combining the functions of ferroxidation and iron chelation enables engineered bacterial cells to accumulate iron in "ultraparamagnetic" macromolecular complexes, allowing these cells to be trapped with magnetic fields and imaged with MRI in vitro and in vivo. We characterize the properties of these cells and complexes using magnetometry, nuclear magnetic resonance, biochemical assays, and computational modeling to elucidate the unique mechanisms and capabilities of this paramagnetic concept.